M. I. Kamal Chickpea, acridine orange, diversity, growth, variability 96-108 7(4) 2023 October 2023 This study aimed to increase genetic diversity in chickpea using acridine orange for improvement of growth and yield traits using diverse genotypes. Six doses of acridine orange were used in this study in addition to the control against one genotype of chickpea. The seeds were planted in randomized complete block design with three replications. Treated populations were evaluated to study the extent of genetic variations induced in growth and yield parameters in M1 generation. Root growth was significantly increased above untreated plants in response to 80 ppm. The dose of 320 ppm generated the maximum concentration in total chlorophyll without any significant increase in carotenoids concentration at any of acridine doses. The dose of 20 ppm recorded significant increase in 100-seed weight above the control. The degree of homogeneity was differed within the same trait and from trait to another in the same genotype. Higher PCV and GCV were exhibited by plant fresh weight, plant dry weight, root length, carotenoids concentration, chlorophyll a, b, total, number of pods per plant and 100-seed weight. This study was highlight into genetic variability induced in chickpea and development of outstanding genotypes.