Abdullah, M. Aslam, F. Gul, M. S. Akram, A. Khadim, A. Sattar, M. Z. U Din Haider, Abdurrehman Line × Tester analysis, correlation, yield, variability, heterosis 149-161 9 (4) 2025 October, 2025 Maize (Zea mays L.) is a staple cereal crop of strategic importance owing to its wide adaptability, short growth cycle and high genetic potential. However, maize productivity in Pakistan remains suboptimal, necessitating the identification of genetically superior hybrids to improve yield. This study was conducted to evaluate the combining ability of maize genotypes for yield and associated traits through Line × Tester analysis. Five inbred lines and five testers were crossed to generate 25 F₁ hybrids, which were evaluated for plant height, ear height, cob length, stem diameter, number of seed rows, 100-grain weight, internodal length and other morphological traits during the 2024 spring season at the University of Agriculture, Faisalabad. Highly significant differences were observed among crosses, lines, testers and their interactions for most traits, indicating substantial genetic variability. Line GB-5 and tester SB-10 exhibited strong general combining ability for key yield traits, whereas crosses such as GB-3 × SB-8 and GB-2 × SB-10 showed high specific combining ability, suggesting non-additive gene action plays a major role in trait expression. The significance of replication effects for traits such as plant height and seed row number confirmed environmental consistency in trait performance. Traits such as cob diameter and number of seed rows were strongly linked with plant and ear height, implying that vegetative vigor contributes to yield potential. Internodal length was positively associated with stem diameter and number of seed rows, highlighting its contribution to reproductive efficiency. These findings emphasize the potential of identified parents and crosses for hybrid development. The genetic diversity and significant GCA and SCA effects reported can guide breeders in selecting promising combinations to enhance maize yield in Pakistanand#39;s agro-climatic conditions.