S.Vanisri crispr cas, plant breeding 1-1 3 (1) 2019 January,2019 Due to advent in technology the plant breeding methods are surging towards identification of the superior genes with accurate precision. Classical and mutational breeding have resulted in significant improvements in agronomic traits, with certain limitations in terms of labour, time and precise knowledge of selection. Marker-aided selection with the help of already identified markers assisted the breeders in accurate selection for those superior traits. Under new breeding techniques (NBTs) CRISPR/Cas (cluster regularly interspaced short palindromic repeats /CRISPR-associated proteins) for genome editing (GE) has great potential for crop improvement. CRISPR has become one of the most powerful gene editing tools today. Unlike other genetic engineering tools, CRISPR is cheap, relatively easy to use and precise.