S. Nimbal, R. K. Behl and A.K. Chhabra Wheat, SDS-PAGE, high molecular weight, Triticum aestivum. 28-31 1 (1) 2017 August,2017 Bread making quality characterization of wheat genotypes is a widely used approach to evaluate breeding material. High Molecular Weight (HMW) glutenin subunits of 27 Indian wheat varieties were determined using SDS-PAGE. A Glu-1 quality was calculated for each variety based on previously observed associations between individual HMW subunits and SDS-sedimentation volume. The results indicated that genotypes WH 423, WH 533, WH 542, PBW 343, UP 2425 and HGPC have highest Glu-1 quality score. These varieties are known to have good bread making quality. HMW glutenin subunits 5+10 controlled by Glu-D1 locus demonstrated the highest effect on bread making quality.