P. Thapa, A. shrestha Protein, soybean, nodule, morphology, diversity 8-17 5 (1) 2021 January, 2021 The present investigation was conducted at Rampur chitwan region of the Napal during 2015. The objectives of the study were to assess the genetic variability in agro-morphological characters of newly developed soybean accessions and to identify the accession with high yield potentials. Fifteen soybean accessions including six released varieties obtained from National Grain legume Research Program, Nepalgunj were carried out in Randomized Complete Block Design (RCBD) with three replications. Number of nodules, days to flowering, days to maturity, plant height, numbers of fruiting nodes/plant, number of branches/plant, numbers of pods/plant, numbers of seeds/plant, seed diameter, test weight and grain yield were recorded. There exists a wide range of variation in agro-morphological characters and yield performance of fifteen soybean accessions. Among the different characters plant height, days to flowering, days to maturity, no of fruiting nodes plant-1 the number of pods plant-1, test weight grain yield and protein content (%) mostly contributed in classifying fifteen accessions into different groups. These characters are important for selecting desirable accession. Accession under cluster 4 i.e. Tarkari-Bhatmas-1 was comparatively early maturing although accessions under cluster 2 were better yielder compared to remaining clusters. The accession Iang-beakong is the best in regards to seed yield (3.36.ton/ha) in that locality.