I. Y. Estouka, M. M. Alhagdow, S. S. Bughrara Ginger, micropropagation, plant growth regulators, activated charcoal, rhizome 106-114 5 (4 ) 2021 October 2021 Simple micropropagation method was established for Zingiber officinale Rosc using fresh rhizome sprouting bud in semisolid culture media. Fresh rhizome sprouting buds were surface sterilized by sodium hypochlorite (2, 2.5, or 3%) for 20 minutes. A concentration of 3% Clorox significantly reduced contamination of rhizome sprouted bud explants, where contamination-free culture was 90%, at the same time; the aseptic explants’ survival rate was high. Plantlets cultured on Murashige and Skoog’s (MS) medium were supplemented with different concentrations (2, 3, 4, or 5 mg/l) of BAP (6-benzyl- amino-purine) with or without 3% AC (activated charcoal), and combinations of BAP (2, or 3 mg/l) with NAA (andalpha;- Naphthalene acetic acid) (0.5, or 1 mg/l) for shoot and root induction. The maximum shoot and root number was obtained from MS medium containing 3 mg/l BAP with a mean of 5.4 shoot per explant, each shoot possesses in mean 10 leaves and 17.7 roots. Plantlets were acclimatization before transplanting, and high survival rate was recorded. Plants would be transferred to the field to produce rhizomes, the useful and economical part of ginger. The success of field culture will open new era of ginger production in Libya.